NeoPlat Blast Mac OS

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Installing BLAST in OS X

These instructions apply to installing BLAST 2.2.22 in OS X, because this is the version that is supported and checked-for by QIIME.

Option 1: Installing BLAST within your user account

Follow these instructions to install BLAST in your home directory. Only do this if you are the only user on your computer. If the computer has multiple users, follow the instructions below for Option 2: Installing BLAST in a central location.
1.Download (but do not unzip) this file, and save in your Downloads folder:
ftp://ftp.ncbi.nlm.nih.gov/blast/executables/release/2.2.22/blast-2.2.22-universal-macosx.tar.gz
2. Unzip it in the Terminal using tar:
cd ~/Downloads
tar -xvf blast-2.2.22-universal-macosx.tar.gz

This should create a new folder called blast-2.2.22
3. Move it somewhere permanent - in this example, we'll just put it in your home directory.
4. Add the ~/blast-2.2.22/bin folder to your PATH using the nano text editor:

nano ~/.profile

This will open the nano text editor, editing a file called .profile in your home directory. Here you can add this line, referencing the directory that contains the BLAST executables:
export PATH=/Users/YourName/blast-2.2.22/bin:${PATH}
where 'YourName' should be your username (or, '/Users/Yourname/' should be your home directory name). If you aren't sure what your home directory is, you can open a new terminal session and type 'cd ~' followed by 'pwd'. You can then save your .profile file in nano by pressing Control-o followed by return to accept the file name. To exit nano, press Control-x.
Close your current Terminal session(s) and open a new one. Now, when you type this command:
it should state the location of the blastall executable file:
/Users/YourName/blast-2.2.22/bin/blastall


5. Make a ~/.ncbirc file that points to the BLAST data directory:
nano ~/.ncbirc

And put this text into the file:

where you should put in your username/home directory instead of 'YourName.' Save the .ncbirc file by pressing Control-o followed by return to accept the file name. To exit nano, press Control-x.
You should now have a functioning copy of BLAST, ready to be called from QIIME or anything else.

Option 2: Installing BLAST in a central location

These instructions will put BLAST in a central location so any user can run BLAST searches. You need to have administrative privileges to do this (i.e., you need to be an sudoer). If you are the only user on your computer, Option 1 may be better.
1.Download (but do not unzip) this file, and save in your Downloads folder:
ftp://ftp.ncbi.nlm.nih.gov/blast/executables/release/2.2.22/blast-2.2.22-universal-macosx.tar.gz
2. Unzip it in the Terminal using tar:
cd ~/Downloads
tar -xvf blast-2.2.22-universal-macosx.tar.gz

This should create a new folder called blast-2.2.22
3. Copy it somewhere permanent - in this example, we'll put it in the /opt directory at the root of the filesystem, so anyone can access the files.
(If the 'mkdir' command says that /opt already exists, this is okay.)

4. Add the /opt/blast-2.2.22/bin folder to your PATH using the nano text editor:

sudo nano /etc/bashrc

This will open the nano text editor, editing a file called /etc/bashrc. Here you can add this line, referencing the directory that contains the BLAST executables:
export PATH=/opt/blast-2.2.22/bin:${PATH}
You can then save the /etc/bashrc file by pressing Control-o followed by return to accept the file name. To exit nano, press Control-x.
Close your current Terminal session and open a new one. Now, when you type this command:
it should state the location of the blastall executable file:
/opt/blast-2.2.22/bin/blastall


5. Make a ~/.ncbirc file that points to the BLAST data directory:
nano ~/.ncbirc

And put this text into the file:

Save the .ncbirc file by pressing Control-o followed by return to accept the file name. To exit nano, press Control-x. EVERY USER needs to do this step in their own home directory. Any user running BLAST needs to have a ~/.ncbirc file.
You should now have a functioning copy of BLAST, ready to be called from QIIME or anything else.

How To Install and Use NCBI Blast+ on Mac

Framerate unlocker mod for Marble Blast Gold - Mac build. Unlock your FPS all the way up to 1000fps. Mileage varies depending on your CPU. Instructions: 1) Unzip the Contents directory 2) Right click on Marble Blast Gold and press Show Package Contents 3) Merge the Contents directory you unzipped with the one in your MBG. Blast doesn't care where the file is located or what you've done with it – quite simply it just monitors your actions and keeps the most recent material available at all times. Dimensionshifter mac os. A neat feature of Blast is the ability to drag a file from its container to anywhere else in the filesystem. 'Worms Blast is a puzzle game similar in concept to Puzzle Bobble, with several important distinctions and gameplay features taken from Worms games. Instead of releasing and matching bubbles, the player has to color blocks on a hexagonal grid. Unlike most Bust-A-Move variants, the player character can move right and left, sailing in a boat.

This page is prepared for the people who want to use NCBI Blast+ on local machines, because we found most of the NCBI Blast+ information available on the Internet is out-dated or inaccurate.
Procedure below is confirmed to work fine on OS X 10.9 Mavericks, 10.10 Yosemite, and 10.11 El Capitan. Risen (itch) mac os. NCBI Blast+ works even on MacBook.
It is assumed that Firefox web browser is installed on your Mac and set as a default browser. If you do not have Firefox, you can download it from here for free. This instruction does not cover all blast derivatives, such as blastx, mega blast or psiblast. If you want to use these blast programs, please refer to http://www.ncbi.nlm.nih.gov/books/NBK1763/. We hope information there is correct.

NCBI Blast+ version here is 2.4.0 released on 6/1/2016. If you prefer newer version of NCBI Blast+, try web-based NCBI Blast+. It support NCBI Blast+ 2.6.0.

Mac OS X LeopardThe Mac OS X Leopard (v10.5) operating system introduces over 300 more features than the previous version including a new desktop, updated Finder, Time Machine, Spaces, Boot Camp and more, delivering a brand new interaction experience, improved usability, enhanced data security and constant simplicity to boost your productivity. A monsters house - ludum dare 33 mac os. Gorilla chief slot.

If you don't understand any part of the instruction below, we recommend you to try BlastStation2 or BlastStation-Local64 to save time. https://softwarethoughts.mystrikingly.com/blog/lots-of-zoms-mac-os. You can start Blast search in less than five minutes with the intuitive manner of operation, amazing easy-to-use interface, and useful extra functions including summary table exporting in CSV format and hit sequence exporting in FASTA format.

1. Installation

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NeoPlat
  • Click the link to download installer from ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/2.4.0/ncbi-blast-2.4.0+.dmg
  • Check 'Open with DiskImageMounter(Default)' and Click OK
  • Double click ncbi-blast-2.4.0+.pkg. If you see the dialog below, Hold down the Control key and click ncbi-blast-2.4.0+.pkg. From the contextual menu choose Open.
  • Follow the instructions of installer
    All program will be installed under '/usr/local/ncbi/blast/bin'
    If you want to uninstall, double click uninstall_ncbi_blast.zip in mounted disk image to extract it and launch it.
    README.txt which contains the link to he BLAST Help web page will be installed in '/usr/local/ncbi/blast/doc'. user_manual.pdf is not bundled with the installer anymore.

2. Setup environments

  • In order to use NCBI Blast+, Blast database file location should be specfied. One option is .ncbirc file.
  • Make .ncbirc text file in your home folder. One example is shown below. Your account is assumed to be MyAccount.
; Start the section for BLAST configuration
[BLAST]
; Specifies the path where BLAST databases are installed
BLASTDB=/Users/MyAccount/blast/db
; Specifies the data sources to use for automatic resolution
; for sequence identifiers
DATA_LOADERS=none
; Specifies the BLAST database to use resolve protein sequences
;BLASTDB_PROT_DATA_LOADER=nr
; Specifies the BLAST database to use resolve protein sequences
;BLASTDB_NUCL_DATA_LOADER=nt
; Windowmasker settings (experimental)
[WINDOW_MASKER]
WINDOW_MASKER_PATH=/Users/MyAccount/blast/db/windowmasker
; end of file
  • Create BLAST database directory.
    1) Open Applications/Utilities/Terminal.
    2) Type 'mkdir -p ~/blast/db' and type return.
  • Installer will install /etc/paths.d/ncbi_blast to set path.

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3. Download database

  • Click the link to visit NCBI ftp server. ftp://ftp.ncbi.nih.gov/blast/db/
  • Double click the database file you want to download, such as nt.00.tar.gz.
  • Save the file in ~/blast/db
  • Open Applications/Utilities/Terminal.
  • Type 'cd ~/blast/db' and type return.
  • Type 'tar xfz nt.00.tar.gz' and type return. nt.00.tar.gz should be the file name you downloaded. Downloaded file will be extracted in ~/blast/db.

4. Create database

  • Download or create FASTA file. File name is assumed to be testdb.fasta.
  • Copy testdb.fasta to ~/blast/db.
  • Open Applications/Utilities/Terminal.
  • Type 'cd ~/blast/db' and type return.
  • Type 'makeblastdb -in testdb.fasta -out testdb -dbtype nucl' for DNA or 'makeblastdb -in testdb.fasta -out testdb -dbtype prot' for Protein and type return to get the database named testdb.
  • Type 'makeblastdb -help' for advanced options.

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5. Launch NCBI-Blast+ commands

  • Create dna or protein query FASTA file. It can contain multi FASTA data. File name is assumed to be test_dna.fasta and test_protein.fasta here. nt.00 is a dna database file and nr.00 is a protein database file available at ftp://ftp.ncbi.nih.gov/blast/db/. These database files are assumed to be downloaded already.
  • blastn
    1) Open Applications/Utilities/Terminal.
    2) Type 'blastn -query test_dna.fasta -db nt.00 -out test.html -html' and type return.
    3) Type 'blastn -help' for advanced options.
  • blastp
    1) Open Applications/Utilities/Terminal.
    2) Type 'blastp -query test_protein.fasta -db nr.00 -out test.html -html' and type return.
    3) Type 'blastn -help' for advanced options.
  • How to use multi cores for Blast search
    1) Add '-num_threads 2' to use 2 cores.




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